Detecting C4d in Liver Allografts: Immunohistochemistry and Immunofluorescence Show Equivalent Staining
Alexis Scherl MD PhD1, Simona Jakab MD2, Gilbert Moeckel MD PhD1, Kisha Mitchell MD1, Dhanpat Jain MD11, Sukru Emre MD3 and Marie Robert MD1
Departments of 1Pathology, 2Medicine and 3Transplant Surgery, Yale School of Medicine, New Haven, CT, USA
BACKGROUND
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Endothelial deposition of C4d, detected by immunofluorescent staining (IF), is the gold standard for the diagnosis of antibody-mediated rejection (AMR) in renal allografts. In contrast, the reliability of C4d stains in liver allografts has yet to be definitively established. The aims of this study are:
- To compare the staining patterns of C4d stains in liver biopsies by IF and immunohistochemistry (IHC)
- To correlate endothelial deposition of C4d with the results of serologic tests for AMR
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