Proteomic Identification of Tissue Degradation Indicators for Tissue Quality Assessment
Kelly Considine, Catherine Kil, Michael C. Stankewich, Bartose Smarkucki, Jie Li, Alexander O. Vortmeyer
Department of Pathology, Yale School of Medicine, New Haven, CT, USA
INTRODUCTION
Analysis of human surgical specimens is important for diagnosis and treatment of disease. Analytical methods include – among others – histologic stains as well as DNA, RNA and protein analysis. However, variable degrees of molecular degradation typically occur in human surgical specimens before the degradation process is effectively suspended via fixation or freezing techniques. Since degradation is known to affect analytical results, a universal test for biochemical integrity would be of great interest for specimen-based studies. We therefore initiated an investigation to identify protein markers for tissue degradation assessment.
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